Journal: bioRxiv

Article Title: Impact of the MX segment on the biogenesis of α 7 nACh receptors

doi: 10.64898/2026.04.02.715926

Figure Lengend Snippet: 1 mM acetylcholine-induced normalized currents elicited from MX-wt, MX-A, MX-AA and MX-AAA constructs expressed alone in Xenopus laevis oocytes or co-expressed with RIC-3 and/or NACHO. Biological replicates are numbers at the top of the chart. Statistical significance was determined using ordinary one-way ANOVA with Tukey’s multiple comparisons test. Statistical significance for MX-wt vs. MX-wt + RIC-3, MX-wt vs. MX-wt + NACHO, and MX-wt + RIC-3 vs. MX-wt + NACHO with P = 3.9 x 10 -14 , P < 10 -10 , and P < 10 -10 , respectively. There was no significant difference in α7 current amplitude when co-expressed with NACHO or both NACHO and RIC-3. The α7 current amplitude elicited from the MX-wt + RIC-3 and MX-wt + NACHO conditions significantly differ from those of the MX-A + RIC-3 and MX-A + NACHO conditions, respectively (P= 0.4 x 10 -10 , P < 10 -11 ).

Article Snippet: Currents of α7 nACh channels were evoked by applying 1 mM acetylcholine chloride (Cat # 159171000, Thermo Scientific Chemicals) in recording buffer.

Techniques: Construct